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gapdh mouse ab  (Bioss)


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    Structured Review

    Bioss gapdh mouse ab
    Gapdh Mouse Ab, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh+antibody/GAPDH+(4F8)+Monoclonal+Antibody/pmc13125192-138-0-4
    Average 95 stars, based on 92 article reviews
    gapdh mouse ab - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes
    Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with anti-Gapdh antibody (1: 1000; #bs-13282R, Bioss), anti-Ucp-1 antibody (1: 500; #bs-1925R, Bioss), anti-GSK-3β antibody (1: 1000; #bs-0023R, Bioss), anti-pGSK-3β antibody (Ser9; 1: 1000, #bs-2066R; Bioss), and anti-β-catenin antibody (Ser9; 1: 1000; #bs-1165R, Bioss), respectively, at 4°C overnight. .. The membrane was next incubated in Tris-buffered saline with Tween 20 with IgG-horseradish peroxidase (1: 8000; no. ZB-2306; Zsbio) at room temperature for 2 h. An enhanced chemiluminescence substrate (Cell Signaling Technology, Danvers, MA, USA) and a chemiluminescence detection system (Promega, Madison, WI, USA) were used to visualize the targeting protein.

    Article Title: WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis
    Article Snippet: .. After blocking with 5% fat-free dry milk at RT for 1 h, the obtained membranes were incubated with an anti-WTAP antibody (#41934S, 1:1000, Cell Signaling Technology, MA, USA), anti-RUNX2 antibody (#12556, 1:1000, Cell Signaling Technology, MA, USA), anti-ALP antibody (#DF6225, 1:1000, Affinity Bioscience, Jiangsu, China), anti-OCN antibody (#bs-0470R, 1:1000, Bioss, Beijing, China), anti-COL1 antibody (#ab270946, 1:1000, Abcam, Cambridge, UK), anti-LPL antibody (#ab91606, 1:1000, Abcam, Cambridge, UK), anti-PPARγ antibody (#2443S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPα antibody (#8178S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPβ antibody (#43095S, 1:1000, Cell Signaling Technology, MA, USA), anti-HDAC4 antibody (#7628, 1:1000, Cell Signaling Technology, MA, USA), anti-MMP-9 antibody (#ab76003, 1:1000, Abcam, Cambridge, UK), anti-NFATc1 antibody (#ab183023, 1:1000, Abcam, Cambridge, UK), anti-CTSK antibody (#ab187647, 1:1000, Abcam, Cambridge, UK), anti-C-FOS antibody (#ab302667, 1:1000, Abcam, Cambridge, UK), and anti-GAPDH antibody (#bsm-33033M, 1:5000, Bioss, Beijing, China) at 4 °C overnight. .. The treated membranes were then incubated with a secondary antibody (#7074S, 1:3000, Cell Signaling Technology, MA, USA).

    Membrane:

    Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes
    Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with anti-Gapdh antibody (1: 1000; #bs-13282R, Bioss), anti-Ucp-1 antibody (1: 500; #bs-1925R, Bioss), anti-GSK-3β antibody (1: 1000; #bs-0023R, Bioss), anti-pGSK-3β antibody (Ser9; 1: 1000, #bs-2066R; Bioss), and anti-β-catenin antibody (Ser9; 1: 1000; #bs-1165R, Bioss), respectively, at 4°C overnight. .. The membrane was next incubated in Tris-buffered saline with Tween 20 with IgG-horseradish peroxidase (1: 8000; no. ZB-2306; Zsbio) at room temperature for 2 h. An enhanced chemiluminescence substrate (Cell Signaling Technology, Danvers, MA, USA) and a chemiluminescence detection system (Promega, Madison, WI, USA) were used to visualize the targeting protein.

    Incubation:

    Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes
    Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with anti-Gapdh antibody (1: 1000; #bs-13282R, Bioss), anti-Ucp-1 antibody (1: 500; #bs-1925R, Bioss), anti-GSK-3β antibody (1: 1000; #bs-0023R, Bioss), anti-pGSK-3β antibody (Ser9; 1: 1000, #bs-2066R; Bioss), and anti-β-catenin antibody (Ser9; 1: 1000; #bs-1165R, Bioss), respectively, at 4°C overnight. .. The membrane was next incubated in Tris-buffered saline with Tween 20 with IgG-horseradish peroxidase (1: 8000; no. ZB-2306; Zsbio) at room temperature for 2 h. An enhanced chemiluminescence substrate (Cell Signaling Technology, Danvers, MA, USA) and a chemiluminescence detection system (Promega, Madison, WI, USA) were used to visualize the targeting protein.

    Article Title: Selective blockade of acid-sensing ion channel 1a can provide substantial hippocampal neuroprotection
    Article Snippet: Protein samples were separated by 10% SDS–polyacrylamide gel and then transferred to polyvinyl difluoride membranes (Millipore, United States), which were then blocked for 1 h with 5% skim milk in TBST [10 mM Tris, 150 mM NaCl, and 0.05% Tween 20 (pH 8.3)] at room temperature. .. The membranes were incubated overnight at 4°C with anti-ASIC1 antibody (Abcam, ab284406, UK) or anti-Rho antibody (Abcam, ab40673, UK), anti-NFATc1 antibody (Abcam, ab177464, UK), anti-LC3B antibody (Abmart, T55992 , China), anti-NFATc4 antibody (CST, 2188, United States), anti-Na+/K+-ATPase antibody (Abbkine, ABL1141, China), anti-COX antibody (Abbkine, ABL1060, China), anti-β-actin antibody (Bioss, bs-0061R, China), and anti-GAPDH antibody (Bioss, bs-10900R, China). .. The membranes were washed in TBST and incubated with a secondary antibody (1:10,000, Bioss, China) for 1 h at room temperature, followed by exposure to electrochemiluminescence.

    Article Title: WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis
    Article Snippet: .. After blocking with 5% fat-free dry milk at RT for 1 h, the obtained membranes were incubated with an anti-WTAP antibody (#41934S, 1:1000, Cell Signaling Technology, MA, USA), anti-RUNX2 antibody (#12556, 1:1000, Cell Signaling Technology, MA, USA), anti-ALP antibody (#DF6225, 1:1000, Affinity Bioscience, Jiangsu, China), anti-OCN antibody (#bs-0470R, 1:1000, Bioss, Beijing, China), anti-COL1 antibody (#ab270946, 1:1000, Abcam, Cambridge, UK), anti-LPL antibody (#ab91606, 1:1000, Abcam, Cambridge, UK), anti-PPARγ antibody (#2443S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPα antibody (#8178S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPβ antibody (#43095S, 1:1000, Cell Signaling Technology, MA, USA), anti-HDAC4 antibody (#7628, 1:1000, Cell Signaling Technology, MA, USA), anti-MMP-9 antibody (#ab76003, 1:1000, Abcam, Cambridge, UK), anti-NFATc1 antibody (#ab183023, 1:1000, Abcam, Cambridge, UK), anti-CTSK antibody (#ab187647, 1:1000, Abcam, Cambridge, UK), anti-C-FOS antibody (#ab302667, 1:1000, Abcam, Cambridge, UK), and anti-GAPDH antibody (#bsm-33033M, 1:5000, Bioss, Beijing, China) at 4 °C overnight. .. The treated membranes were then incubated with a secondary antibody (#7074S, 1:3000, Cell Signaling Technology, MA, USA).

    Saline:

    Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes
    Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with anti-Gapdh antibody (1: 1000; #bs-13282R, Bioss), anti-Ucp-1 antibody (1: 500; #bs-1925R, Bioss), anti-GSK-3β antibody (1: 1000; #bs-0023R, Bioss), anti-pGSK-3β antibody (Ser9; 1: 1000, #bs-2066R; Bioss), and anti-β-catenin antibody (Ser9; 1: 1000; #bs-1165R, Bioss), respectively, at 4°C overnight. .. The membrane was next incubated in Tris-buffered saline with Tween 20 with IgG-horseradish peroxidase (1: 8000; no. ZB-2306; Zsbio) at room temperature for 2 h. An enhanced chemiluminescence substrate (Cell Signaling Technology, Danvers, MA, USA) and a chemiluminescence detection system (Promega, Madison, WI, USA) were used to visualize the targeting protein.

    SDS Page:

    Article Title: Lysine 222 in PPAR γ1 functions as the key site of MuRF2-mediated ubiquitination modification
    Article Snippet: .. Bound proteins were then eluted by boiling in 1× SDS loading buffer, resolved on a 6% Bis-Tris gel with SDS-PAGE running buffer and transferred to PVDF membranes for immunoblotting with goat anti-MuRF2 antibody (Abcam, Cat. #Ab4387), rabbit anti-PPAR γ1 antibody (Cell Signaling Technology, Cat. #2443), rabbit anti‐GAPDH antibody (BIOSS, Cat. #bs‐2188R), mouse anti‐His antibody (Beyotime, Cat. #AF5060), mouse anti‐ubiquitin antibody (ENZO, Cat. #BML-UW9920). .. FITC-labeled rabbit anti-goat IgG (H + L) (ZSGB-BIO, Cat. #ZF-0314), horseradish peroxidase‐labeled anti‐rabbit secondary antibody (Abbkine, Cat. #A21020) and horseradish peroxidase‐labeled anti‐mouse secondary antibody (Beyotime, Cat. #A0216) were used to detect the primary antibodies.

    Western Blot:

    Article Title: Lysine 222 in PPAR γ1 functions as the key site of MuRF2-mediated ubiquitination modification
    Article Snippet: .. Bound proteins were then eluted by boiling in 1× SDS loading buffer, resolved on a 6% Bis-Tris gel with SDS-PAGE running buffer and transferred to PVDF membranes for immunoblotting with goat anti-MuRF2 antibody (Abcam, Cat. #Ab4387), rabbit anti-PPAR γ1 antibody (Cell Signaling Technology, Cat. #2443), rabbit anti‐GAPDH antibody (BIOSS, Cat. #bs‐2188R), mouse anti‐His antibody (Beyotime, Cat. #AF5060), mouse anti‐ubiquitin antibody (ENZO, Cat. #BML-UW9920). .. FITC-labeled rabbit anti-goat IgG (H + L) (ZSGB-BIO, Cat. #ZF-0314), horseradish peroxidase‐labeled anti‐rabbit secondary antibody (Abbkine, Cat. #A21020) and horseradish peroxidase‐labeled anti‐mouse secondary antibody (Beyotime, Cat. #A0216) were used to detect the primary antibodies.

    Article Title: Screening of differentially expressed miRNAs during osteogenic/odontogenic differentiation of human dental pulp stem cells exposed to mechanical stress
    Article Snippet: .. Anti-DSPP antibody (bs-10316R, Bioss, 1:500 dilution), anti-DMP1 antibody (bs-12359R, Bioss, 1:500 dilution), anti-RUNX2 antibody (ab23981, Abcam, 1:1000 dilution), anti-GAPDH antibody (bs-12257R, Bioss, 1:5000 dilution), and Goat Anti-Rabbit IgG Secondary Antibody (BA1054, Bioss, 1:2000 dilution) were used for immunoblot assays. ..

    Article Title: Adipocyte-secreted PRELP promotes adipocyte differentiation and adipose tissue fibrosis by binding with p75 NTR to activate FAK/MAPK signaling.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    Image Search Results


    Senescent Microenvironment-Educated Mesenchymal Stem Cells Release High-Affinity Senescent NPC Domesticated Extracellular Vesicles. (A) Schematic diagram of the experimental setup for educating MSCs with SASP-CM to generate D-EVs versus N-EVs. (B) Confocal microscopy images showing different EVs internalization by senescent NPCs after 12 h in vitro. (C) Flow cytometry and quantification analysis of different EVs uptake by senescent NPCs. (D) In vivo validation of the senescent niche. Representative fluorescence images following injection of senescence-tracer (Red). (E) In vivo PKH26-labeled D-EVs tracking. (F) Representative SA-β-Gal images and quantification of MSCs treated with SASP-CM or not. (G) Gene Ontology (GO) analysis confirming enrichment of external encapsulating structure organization and cytokine production in Biological Process (BP) categories. (H) Heatmap indicating gene expression associated with EVs biogenesis within D-MSCs and N-MSCs. (I) Heatmap indicating gene expression associated with cytokine production within D-MSCs and N-MSCs. (J and L) Gene Ontology (GO) analysis confirming enrichment of terms related to vesicle organization and transport in the Cellular Component (CC) categories. (K) Western blot analysis confirmed core senescence markers p16 and p21 and DNA damage marker γ-H2AX in N-MSC and D-MSC. (M) Western blot analysis confirmed the expression of CD9, CD63, TSG101, Calnexin, and GM130 in MSC-EVs, N-EVs, or D-EVs. (N) TEM images showing the morphology and size of MSC-derived EVs, N-EVs, and D-EVs. (O) NTA shows size distribution in MSC-EVs, N-EVs, or D-EVs. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: Senescent Microenvironment-Educated Mesenchymal Stem Cells Release High-Affinity Senescent NPC Domesticated Extracellular Vesicles. (A) Schematic diagram of the experimental setup for educating MSCs with SASP-CM to generate D-EVs versus N-EVs. (B) Confocal microscopy images showing different EVs internalization by senescent NPCs after 12 h in vitro. (C) Flow cytometry and quantification analysis of different EVs uptake by senescent NPCs. (D) In vivo validation of the senescent niche. Representative fluorescence images following injection of senescence-tracer (Red). (E) In vivo PKH26-labeled D-EVs tracking. (F) Representative SA-β-Gal images and quantification of MSCs treated with SASP-CM or not. (G) Gene Ontology (GO) analysis confirming enrichment of external encapsulating structure organization and cytokine production in Biological Process (BP) categories. (H) Heatmap indicating gene expression associated with EVs biogenesis within D-MSCs and N-MSCs. (I) Heatmap indicating gene expression associated with cytokine production within D-MSCs and N-MSCs. (J and L) Gene Ontology (GO) analysis confirming enrichment of terms related to vesicle organization and transport in the Cellular Component (CC) categories. (K) Western blot analysis confirmed core senescence markers p16 and p21 and DNA damage marker γ-H2AX in N-MSC and D-MSC. (M) Western blot analysis confirmed the expression of CD9, CD63, TSG101, Calnexin, and GM130 in MSC-EVs, N-EVs, or D-EVs. (N) TEM images showing the morphology and size of MSC-derived EVs, N-EVs, and D-EVs. (O) NTA shows size distribution in MSC-EVs, N-EVs, or D-EVs. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against GAPDH (1:5000, 104941-AP, Proteintech), TSG101 (1:1000, DF8427, Affinity), CD9 (1:1000, AF5139, Affinity), CD63 (1:2000, 25682-1-AP, Proteintech), Calnexin (1:5000, 10427-2-AP, Proteintech), GM130 (1:20000, 11308-1-AP, Proteintech), CXCR3 (1:5000, 26756-1-AP, Proteintech), CXCL10 (1:2000, 10937-1-AP, Proteintech), MMP3 (1:2000, 17873-1-AP, Proteintech), ADAMTS5 (DF13268, Affinity), P16 (AF5484, Affinity), P21 (10355-1-AP, Proteintech), GPX4 (1:1000, 381958, Zen-bio), SLC7A11 (1:1000, 26864-1-AP, Proteintech), ACSL4 (1:5000, 22401-1-AP, Proteintech) and Tubulin (1:10000, T40103 , Abmart) overnight at 4 °C.

    Techniques: Confocal Microscopy, In Vitro, Flow Cytometry, In Vivo, Biomarker Discovery, Fluorescence, Injection, Labeling, Gene Expression, Western Blot, Marker, Expressing, Derivative Assay