gapdh mouse ab (Bioss)
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Gapdh Mouse Ab, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gapdh+antibody/GAPDH+(4F8)+Monoclonal+Antibody/pmc13125192-138-0-4
Average 95 stars, based on 92 article reviews
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Blocking Assay:Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with Article Title: WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis Article Snippet: .. After blocking with 5% fat-free dry milk at RT for 1 h, the obtained membranes were incubated with an anti-WTAP antibody (#41934S, 1:1000, Cell Signaling Technology, MA, USA), anti-RUNX2 antibody (#12556, 1:1000, Cell Signaling Technology, MA, USA), anti-ALP antibody (#DF6225, 1:1000, Affinity Bioscience, Jiangsu, China), anti-OCN antibody (#bs-0470R, 1:1000, Bioss, Beijing, China), anti-COL1 antibody (#ab270946, 1:1000, Abcam, Cambridge, UK), anti-LPL antibody (#ab91606, 1:1000, Abcam, Cambridge, UK), anti-PPARγ antibody (#2443S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPα antibody (#8178S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPβ antibody (#43095S, 1:1000, Cell Signaling Technology, MA, USA), anti-HDAC4 antibody (#7628, 1:1000, Cell Signaling Technology, MA, USA), anti-MMP-9 antibody (#ab76003, 1:1000, Abcam, Cambridge, UK), anti-NFATc1 antibody (#ab183023, 1:1000, Abcam, Cambridge, UK), anti-CTSK antibody (#ab187647, 1:1000, Abcam, Cambridge, UK), anti-C-FOS antibody (#ab302667, 1:1000, Abcam, Cambridge, UK), and Membrane:Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with Incubation:Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with Article Title: Selective blockade of acid-sensing ion channel 1a can provide substantial hippocampal neuroprotection Article Snippet: Protein samples were separated by 10% SDS–polyacrylamide gel and then transferred to polyvinyl difluoride membranes (Millipore, United States), which were then blocked for 1 h with 5% skim milk in TBST [10 mM Tris, 150 mM NaCl, and 0.05% Tween 20 (pH 8.3)] at room temperature. .. The membranes were incubated overnight at 4°C with anti-ASIC1 antibody (Abcam, ab284406, UK) or anti-Rho antibody (Abcam, ab40673, UK), anti-NFATc1 antibody (Abcam, ab177464, UK), anti-LC3B antibody (Abmart, T55992 , China), anti-NFATc4 antibody (CST, 2188, United States), anti-Na+/K+-ATPase antibody (Abbkine, ABL1141, China), anti-COX antibody (Abbkine, ABL1060, China), anti-β-actin antibody (Bioss, bs-0061R, China), and Article Title: WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis Article Snippet: .. After blocking with 5% fat-free dry milk at RT for 1 h, the obtained membranes were incubated with an anti-WTAP antibody (#41934S, 1:1000, Cell Signaling Technology, MA, USA), anti-RUNX2 antibody (#12556, 1:1000, Cell Signaling Technology, MA, USA), anti-ALP antibody (#DF6225, 1:1000, Affinity Bioscience, Jiangsu, China), anti-OCN antibody (#bs-0470R, 1:1000, Bioss, Beijing, China), anti-COL1 antibody (#ab270946, 1:1000, Abcam, Cambridge, UK), anti-LPL antibody (#ab91606, 1:1000, Abcam, Cambridge, UK), anti-PPARγ antibody (#2443S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPα antibody (#8178S, 1:1000, Cell Signaling Technology, MA, USA), anti-C/EBPβ antibody (#43095S, 1:1000, Cell Signaling Technology, MA, USA), anti-HDAC4 antibody (#7628, 1:1000, Cell Signaling Technology, MA, USA), anti-MMP-9 antibody (#ab76003, 1:1000, Abcam, Cambridge, UK), anti-NFATc1 antibody (#ab183023, 1:1000, Abcam, Cambridge, UK), anti-CTSK antibody (#ab187647, 1:1000, Abcam, Cambridge, UK), anti-C-FOS antibody (#ab302667, 1:1000, Abcam, Cambridge, UK), and Saline:Article Title: Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/β-Catenin Signaling in 3T3-L1 Adipocytes Article Snippet: .. After blocking, the membrane was incubated in Tris-buffered saline with Tween 20 with SDS Page:Article Title: Lysine 222 in PPAR γ1 functions as the key site of MuRF2-mediated ubiquitination modification Article Snippet: .. Bound proteins were then eluted by boiling in 1× SDS loading buffer, resolved on a 6% Bis-Tris gel with SDS-PAGE running buffer and transferred to PVDF membranes for immunoblotting with goat anti-MuRF2 antibody (Abcam, Cat. #Ab4387), rabbit anti-PPAR γ1 antibody (Cell Signaling Technology, Cat. #2443), rabbit Western Blot:Article Title: Lysine 222 in PPAR γ1 functions as the key site of MuRF2-mediated ubiquitination modification Article Snippet: .. Bound proteins were then eluted by boiling in 1× SDS loading buffer, resolved on a 6% Bis-Tris gel with SDS-PAGE running buffer and transferred to PVDF membranes for immunoblotting with goat anti-MuRF2 antibody (Abcam, Cat. #Ab4387), rabbit anti-PPAR γ1 antibody (Cell Signaling Technology, Cat. #2443), rabbit Article Title: Screening of differentially expressed miRNAs during osteogenic/odontogenic differentiation of human dental pulp stem cells exposed to mechanical stress Article Snippet: .. Anti-DSPP antibody (bs-10316R, Bioss, 1:500 dilution), anti-DMP1 antibody (bs-12359R, Bioss, 1:500 dilution), anti-RUNX2 antibody (ab23981, Abcam, 1:1000 dilution), Article Title: Adipocyte-secreted PRELP promotes adipocyte differentiation and adipose tissue fibrosis by binding with p75 NTR to activate FAK/MAPK signaling. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. |
